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kapa low throughput library preparation kit standard  (Roche)


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    Structured Review

    Roche kapa low throughput library preparation kit standard
    Kapa Low Throughput Library Preparation Kit Standard, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kapa+low+throughput+library+preparation+kit+standard/KAPA+HiFi+Kit/pm41135521-264-6-12
    Average 99 stars, based on 5 article reviews
    kapa low throughput library preparation kit standard - by Bioz Stars, 2026-10
    99/100 stars

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    Amplification:

    Article Title: RAF-independent MEK mutations drive refractory histiocytic neoplasms but respond to ERK inhibition.
    Article Snippet: 58,59 Approximately 250 ng of DNA from each sample was sheared to an average of 150 bp in a Covaris instrument for 360 seconds (duty cycle, 10%; intensity, 5; cycles/burst, 200). .. Bar-coded libraries were prepared using the Kapa Low-Throughput Library Preparation Kit Standard (Kapa Biosystems), amplified using the KAPA HiFi Library Amplification Kit (Kapa Biosystems; 8 cycles), and quantified using Qubit Fluorimetric Quantitation (Invitrogen) and Agilent Bioanalyzer. .. An equimolar pool of the 4 bar-coded libraries (300 ng each) was used as input to capture the exome using one reaction tube of the Nimblegen SeqCap EZ Human Exome Library v3.0 (Roche; cat no. 06465684001), according to the manufacturer’s protocol.

    Article Title: Recurrent homozygous deletion of DROSHA and microduplication of PDE4DIP in pineoblastoma.
    Article Snippet: Exome sequencing: 250 ng of DNA from each sample were sheared on a Covaris instrument for 360 s (Duty cycle − 10%; intensity − 5; cycles/Burst − 200). .. Barcoded libraries were prepared using the Kapa Low-Throughput Library Preparation Kit Standard (Kapa Biosystems), amplified using the KAPA HiFi Library Amplification kit (Kapa Biosystems) (eight cycles) and quantified using Qubit Fluorimetric Quantitation (Invitrogen) and Agilent Bioanalyzer. .. An equimolar pool of the four barcoded libraries (300 ng each) was used as input to capture the exome using one reaction tube of the Nimblegen SeqCap EZ Human Exome Library v3.0 (Roche, cat # 06465684001), according to the manufacturer’s protocol.

    Article Title: Recurrent homozygous deletion of DROSHA and microduplication of PDE4DIP in pineoblastoma.
    Article Snippet: The pooled capture library was quantified by Qubit (Invitrogen) and Bioanalyzer (Agilent), and sequenced on an Illumina HiSeq 2500 using a paired end, 100 nucleotides in length run mode to achieve an average of 100× coverage. .. Whole-genome sequencing: 500 ng of DNA from each sample were sheared on a Covaris instrument as described above, and barcoded libraries were prepared using the Kapa Low-Throughput Library Preparation Kit Standard (Kapa Biosystems), without any PCR amplification. ..

    Library Amplification:

    Article Title: RAF-independent MEK mutations drive refractory histiocytic neoplasms but respond to ERK inhibition.
    Article Snippet: 58,59 Approximately 250 ng of DNA from each sample was sheared to an average of 150 bp in a Covaris instrument for 360 seconds (duty cycle, 10%; intensity, 5; cycles/burst, 200). .. Bar-coded libraries were prepared using the Kapa Low-Throughput Library Preparation Kit Standard (Kapa Biosystems), amplified using the KAPA HiFi Library Amplification Kit (Kapa Biosystems; 8 cycles), and quantified using Qubit Fluorimetric Quantitation (Invitrogen) and Agilent Bioanalyzer. .. An equimolar pool of the 4 bar-coded libraries (300 ng each) was used as input to capture the exome using one reaction tube of the Nimblegen SeqCap EZ Human Exome Library v3.0 (Roche; cat no. 06465684001), according to the manufacturer’s protocol.

    Article Title: Recurrent homozygous deletion of DROSHA and microduplication of PDE4DIP in pineoblastoma.
    Article Snippet: Exome sequencing: 250 ng of DNA from each sample were sheared on a Covaris instrument for 360 s (Duty cycle − 10%; intensity − 5; cycles/Burst − 200). .. Barcoded libraries were prepared using the Kapa Low-Throughput Library Preparation Kit Standard (Kapa Biosystems), amplified using the KAPA HiFi Library Amplification kit (Kapa Biosystems) (eight cycles) and quantified using Qubit Fluorimetric Quantitation (Invitrogen) and Agilent Bioanalyzer. .. An equimolar pool of the four barcoded libraries (300 ng each) was used as input to capture the exome using one reaction tube of the Nimblegen SeqCap EZ Human Exome Library v3.0 (Roche, cat # 06465684001), according to the manufacturer’s protocol.

    Quantitation Assay:

    Article Title: RAF-independent MEK mutations drive refractory histiocytic neoplasms but respond to ERK inhibition.
    Article Snippet: 58,59 Approximately 250 ng of DNA from each sample was sheared to an average of 150 bp in a Covaris instrument for 360 seconds (duty cycle, 10%; intensity, 5; cycles/burst, 200). .. Bar-coded libraries were prepared using the Kapa Low-Throughput Library Preparation Kit Standard (Kapa Biosystems), amplified using the KAPA HiFi Library Amplification Kit (Kapa Biosystems; 8 cycles), and quantified using Qubit Fluorimetric Quantitation (Invitrogen) and Agilent Bioanalyzer. .. An equimolar pool of the 4 bar-coded libraries (300 ng each) was used as input to capture the exome using one reaction tube of the Nimblegen SeqCap EZ Human Exome Library v3.0 (Roche; cat no. 06465684001), according to the manufacturer’s protocol.

    Article Title: Recurrent homozygous deletion of DROSHA and microduplication of PDE4DIP in pineoblastoma.
    Article Snippet: Exome sequencing: 250 ng of DNA from each sample were sheared on a Covaris instrument for 360 s (Duty cycle − 10%; intensity − 5; cycles/Burst − 200). .. Barcoded libraries were prepared using the Kapa Low-Throughput Library Preparation Kit Standard (Kapa Biosystems), amplified using the KAPA HiFi Library Amplification kit (Kapa Biosystems) (eight cycles) and quantified using Qubit Fluorimetric Quantitation (Invitrogen) and Agilent Bioanalyzer. .. An equimolar pool of the four barcoded libraries (300 ng each) was used as input to capture the exome using one reaction tube of the Nimblegen SeqCap EZ Human Exome Library v3.0 (Roche, cat # 06465684001), according to the manufacturer’s protocol.

    Sequencing:

    Article Title: Recurrent homozygous deletion of DROSHA and microduplication of PDE4DIP in pineoblastoma.
    Article Snippet: The pooled capture library was quantified by Qubit (Invitrogen) and Bioanalyzer (Agilent), and sequenced on an Illumina HiSeq 2500 using a paired end, 100 nucleotides in length run mode to achieve an average of 100× coverage. .. Whole-genome sequencing: 500 ng of DNA from each sample were sheared on a Covaris instrument as described above, and barcoded libraries were prepared using the Kapa Low-Throughput Library Preparation Kit Standard (Kapa Biosystems), without any PCR amplification. ..

    Polymerase Chain Reaction:

    Article Title: Recurrent homozygous deletion of DROSHA and microduplication of PDE4DIP in pineoblastoma.
    Article Snippet: The pooled capture library was quantified by Qubit (Invitrogen) and Bioanalyzer (Agilent), and sequenced on an Illumina HiSeq 2500 using a paired end, 100 nucleotides in length run mode to achieve an average of 100× coverage. .. Whole-genome sequencing: 500 ng of DNA from each sample were sheared on a Covaris instrument as described above, and barcoded libraries were prepared using the Kapa Low-Throughput Library Preparation Kit Standard (Kapa Biosystems), without any PCR amplification. ..



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